WebSep 1, 1990 · Alternate positioning of such peptides in the carboxy end of the CTB protein was found to assist in antibody recognition and is proposed as a means to help exposure of some foreign epitopes by CTB fusion proteins. The results may be of significance for the development of recombinant oral vaccines based on gene fusions to CTB or to the … WebNational Center for Biotechnology Information

Vaccines Free Full-Text Cholera Toxin Subunit B as …

WebFeb 5, 2001 · Abstract Cholera toxin B subunit (CTB) mature protein was stably expressed in transgenic tobacco plants under the control of the CaMV 35S promoter and TMV Ω fragment. Fusion of the PR1b signal pept... WebDec 5, 2012 · N-Glycosylation analysis of CTB fusion proteins expressed in plants has been performed in a few studies , revealing that the expression of CTB fusion proteins with a C-terminal ER-retention signal, (SE)KDEL, was an effective system for the suppression of β1,2-xylose and/or α1,3-fucose modification on N-glycans. phoenix startup+

N -Glycosylation and N -Glycan Moieties of CTB Expressed in

WebDec 28, 2012 · Since the CTB subunit was first chosen as a carrier protein in 1984 , CTB fusion proteins with insulin , insulin/GAD , and the full-length rotavirus NSP4 were expressed in transgenic potato plants, with the B chain of human insulin expressed in transgenic tobacco plants . More recently, ... WebThe formation of dimers, trimers, tetramers and pentamers of the CTB-Pins fusion protein was also observed (Figure 4a,b). A similar banding pattern was observed by immunodetection with CTB polyclonal antibody (Figure 4c). Quantification of the fusion protein on western blots was performed by comparing plant samples with known … WebCTB Fusion Protein Immunization Increases Aortic FoxP3 and IL-10 mRNA Levels Real-time RT-PCR analysis of the thoracic aorta of apoe / mice showed significant increases … phoenix state correctional institute