WebCTCF was measured for whole cells and separately for apoplast (CTCF apop) regions of the cell. For each combination the cell CTCF and CTCF apop, 30 selected areas of each sample were analyzed based on 50 photos. The levels of fluorescence signal were measured using Fiji (version 2.9.0/1.53 t; National Institutes of Health, Bethesda, MD, USA). WebCTCF is a nuclear protein initially discovered for its role in enhancer-promoter insulation. It has been shown to play a role in genome architecture and in fact, its DNA binding sites are enriched at the borders of chromatin domains. Recently, we showed that depletion of CTCF impairs the DNA damage response to ionizing radiation. To investigate the relationship …
CTCF organizes inter-A compartment interactions through RYBP
WebMar 16, 2024 · Within a few hours of auxin treatment, CTCF expression was undetectable by western blot, single-cell fluorescence imaging or flow cytometry (>99% depletion) (Fig. 3a and Extended Data Fig. 4a–d). WebFinally, live-cell imaging, fluorescence recovery after photobleaching, and single molecule tracking showed that almost all CTCF chromatin binding is lost in prometaphase. Combined, our results demonstrate loss of CTCF binding to CTCF sites during prometaphase and rearrangement of the chromatin landscape around CTCF motifs. grandfather passage spanish
Identification of the elementary structural units of the DNA
WebAug 11, 2024 · Here, we show that the CCCTC-binding factor (CTCF) is a barrier for 2C-like reprogramming. Indeed, forced conversion to a 2C-like state by the transcription factor DUX is associated with DNA ... WebJun 29, 2024 · Results from fluorescence recovery after photobleaching (FRAP) further revealed that large CTCF puncta rapidly recovered fluorescence within 1 min, while the smaller puncta were less efficient in ... WebThe BioTek Cytation 5 cell imaging multimode reader combines automated digital widefield microscopy with conventional multimode microplate reading. Fluorescence, brightfield, color brightfield, and phase contrast imaging are available with the automated cell imager. Fluorescence, luminescence, and UV-Vis absorbance multimode plate reading … chinese chicken and broccoli with brown sauce