WebbGibco™ Zeocin™ Selection Antibiotic is a formulation of phleomycin D1, a glycopeptide antibiotic produced by Streptomyces verticillus; Zeocin™ Selection Antibiotic acts by … WebbA proof reading polymerase (Phusion ® High-Fidelity DNA Polymerase, Thermo Scientific) was used according to the manufacturer’s instructions (50 µL of master mix: 1 µL of forward and reverse primers (0.5 µM each), 10 µL of Phusion HF buffer, 1 µL dNTPs (200 µM), 0.5 µL Phusion DNA polymerase, 24.5 µL of Milli-Q water and 2 µL of DNA).

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Webb6 feb. 2024 · Then the pTE11-Lcc1 construct was transformed into protoplasts of Gps1 WT strain and finally, positive colonies were screened under the selection of zeocin (100 μg/mL) on PDA plates. For generating Lcc1 complemented strains, the pTE11- Lcc1 construct was transformed into protoplasts of Δ Lcc1 -A19 strain and finally positive … WebbXylanases (EC 3.2.1.8) hydrolyze the hemicellulose of plant cell walls. Xylanases are used in the food and paper industries and for bioconversion of lignocellulose to biofuel. In this work, the producer-strain with four copies of the xAor xylanase gene was organized in two tandem copies for optimal expression in Komagataella phaffii T07 yeast. The secreted … middletown used to have a sears

pZeoSV2(+) or pZeoSV2(-)

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