WebMar 1, 2012 · Five different staining techniques were used to test for pollen viability. (i) 1% aqueous solution of 2,3,5-triphenyl tetrazolium chloride (TTC) and (ii) 2,5-diphenyl monotetrazolium bromide ... TTC did not stain dead pollen but IKI stained the dead pollen in a similar manner to fresh pollen (Huang et al., 2004). Pollen viability in ... WebMay 24, 2013 · Infarct volume was determined by 2,3,5-triphenyl-tetrazolium chloride (TTC) staining. Rats were anesthetized with chloral hydrate (10%) after three-days’ exercise. Brains were immediately removed and sectioned into 2 mm coronal slices. Six consecutive sections were incubated in TTC solution (2% TTC in PBS) at 37 °C for 30 min and then …
In Vitro Pollen Viability and Pollen Germination in Cherry Laurel ...
WebMusk is commonly used in the prevention of myocardial infarction and ischemic stroke, and muscone is its main active component.The effect and mechanism of muscone to improve the condition of ischemic stroke is not clear, accordingly, we verified its efficacy in ischemia-reperfused rats, and investigated its mechanism by PC12 and THP-1 cells.A ... WebJul 26, 2024 · 2.3. 2,3,5-Triphenyl-Tetrazolium Chloride (TTC) and Evans Blue Staining Infarct volume was analyzed with TTC staining after photothrombotic stroke and middle cerebral artery occlusion (MCAO) surgery. After the mice were euthanized, the brains were rapidly isolated and frozen, and then the tissue was sectioned into 2 mm-thick coronal … trellis on wall
17779 TTC Solution 1% - Sigma-Aldrich
Web2,3,5-Triphenyl-tetrazolium chloride (TTC) solution is a redox indicator that can help indicate cellular respiration and identify viable and non-viable cells as well as identify … WebImmunohistochemical staining of AC wall is described in a few cases. Studying five cases of AC stained for epithelial membrane antigen (EMA), glial fibrillary acidic protein (GFAP), carcinoembryonic antigen (CEA), S-100, and prealbumin, Inoue et al. found ACs to only be positive for EMA [12]. WebTo confirm infarct severity ex-vivo, the excised heart was divided into 4 axial slices and stained using 1% triphenyl-tetrazolium-chloride (TTC) solution (A–D). The TTC staining in … temperature of refrigerated milk